PROCESS

Consult with specialist

Begin with a thorough consultation with a fertility specialist to discuss your medical history, evaluate your fertility status, and develop a personalized treatment plan.

Ovarian Stimulation

Medications are administered to stimulate the ovaries to produce multiple eggs, increasing the chances of successful fertilization.

Egg retrieval

Mature eggs are collected from the ovaries through a minor surgical procedure, typically performed under ultrasound guidance.

Sperm Collection

A sperm sample is provided by the partner or a donor, which is then prepared for the ICSI procedure.

 

Blastocyst culture

The fertilized eggs (embryos) are cultured in the lab until they reach the blastocyst stage, which usually takes about 5-6 days.

Next-generation sequencing (NGS)

Genetic testing is performed on the embryos to ensure they are healthy and free of specific genetic conditions before transfer.

Uterus preparation

The uterus is prepared with hormonal treatments to create an optimal environment for embryo implantation.

Embryo Transfer

Selected healthy embryos are transferred into the uterus, a quick and painless procedure usually performed under ultrasound guidance.

Pregnancy test

A blood test is conducted approximately two weeks after the embryo transfer to determine if pregnancy has been achieved.

PRP

Steps for PRP Injection into the Endometrium


Pre-injection Preparation

  • The patient needs to undergo a health check and blood tests to ensure their body is suitable for PRP injection.
  • According to the doctor's advice, fasting or discontinuing certain medications may be required.


Blood Collection

  • The doctor will collect approximately 10-20 milliliters of blood from the patient.
  • The collected blood will be separated using a centrifuge to extract platelet-rich plasma.


Preparation of PRP

  • The platelet-rich plasma will be separated and prepared for injection.
  • PRP will be placed into a clean, sterile syringe.


PRP Injection

  • PRP injections are usually administered during the phase when the endometrium is prepared for embryo implantation.
  • The doctor will use special instruments to inject PRP into the endometrium, typically through the vagina and uterus.
  • PRP helps to increase the thickness of the endometrium, making it more suitable for receiving and supporting the implantation of the embryo.


Post-injection Monitoring and Recovery

  • After the injection, the patient will be monitored to prevent infections or other complications.
  • The patient may experience slight discomfort or minor bleeding, but recovery is usually quick.


PRP Administration before Progesterone Use

  • PRP injections can be administered up to three times before starting the use of progesterone medications.
  • Progesterone medications will be used to further promote the preparation of the endometrium, enhancing the chances of successful embryo implantation.


Combined Use with IVF

  • PRP injections are typically used in conjunction with the IVF process, administered during the preparation phase for embryo implantation to increase the chances of successful implantation and development.

Step-by-Step Process of Ovarian PRP


Consultation and Evaluation

  • The patient undergoes a thorough medical evaluation, including a review of medical history, hormonal tests, and ultrasound assessments to determine ovarian reserve and overall reproductive health.


Blood Draw

  • A sample of the patient’s blood is drawn, usually from a vein in the arm. The volume of blood collected can vary but typically ranges from 20 to 60 milliliters.


Preparation of PRP

  • The blood sample is processed using a centrifuge, a machine that spins the blood at high speeds to separate its components. This process isolates the platelet-rich plasma from the other blood components (red blood cells and white blood cells).
  • The resulting PRP is a concentrated solution rich in platelets and growth factors essential for tissue repair and regeneration.


Activation of PRP

  • In some protocols, PRP may be activated by adding substances like calcium chloride or thrombin to release growth factors more effectively.


Ultrasound-Guided Injection

  • The PRP is injected into the ovaries using a fine needle. This procedure is performed under ultrasound guidance to ensure accurate placement of the PRP into the ovarian tissue.
  • Local anesthesia or mild sedation may be used to minimize discomfort during the injection.


Post-Procedure Monitoring

  • After the injection, the patient is monitored for a short period to ensure there are no immediate adverse reactions. Most patients can return to normal activities shortly after the procedure.
  • Follow-up visits may be scheduled to monitor ovarian response through blood tests and ultrasound examinations.


Potential Repeat Treatments

  • Depending on the patient’s response, additional PRP treatments may be recommended. The interval between treatments and the total number of sessions vary based on individual cases and treatment protocols.

sperm washing

Steps of Sperm Washing

Collecting the Sperm Sample

The sperm sample is collected from the male, either through ejaculation or extraction from the testes in some cases.

Preparing the Washing Solution

The washing solution used in sperm washing consists of safe, non-toxic solutions such as HEPES buffer or culture media.

Mixing the Sperm Sample with the Solution

The sperm sample is mixed with the washing solution.

Centrifugation

The mixture is then centrifuged to separate high-quality sperm from unwanted cells and substances.

Collecting High-Quality Sperm

The separated high-quality sperm are collected and washed again with the solution to remove any remaining impurities.

Quality Assessment

The washed sperm are evaluated to ensure that they are of good quality and ready for use in fertilization procedures.

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